FastQCFastQC Report
Thu 15 Oct 2020
HNTYJDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTYJDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990417355
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT207280900.082.091781
AGCAGTG228462000.074.456552
GCAGTGG263019150.064.675533
CAGTGGT278064850.061.1293954
AGTGGTA284561950.059.7284135
GTGGTAT300203100.056.5797586
TGGTATC301417000.056.327637
GGTATCA304173200.055.8326958
GTATCAA306183050.055.483889
CAACGCA306380800.030.86575512-13
ATCAACG306535100.030.8387610-11
ACGCAGA308011900.030.69785514-15
AGAGTAC307564150.030.58338718-19
AGTACAT306421650.030.57104320-21
GCAGAGT309081250.030.51532716-17
TACATGG306705150.030.4907422-23
CATGGGG111612850.029.86372824-25
CATGGGC53817550.029.05661224-25
CATGGGT75378900.028.9375924-25
CATGGGA78153000.027.97829424-25
Produced by FastQC (version 0.11.5)