FastQCFastQC Report
Thu 15 Oct 2020
HNTYJDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTYJDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990417355
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTTAT936583789.456455657524296No Hit
CCACAGGG934547889.435899676858954No Hit
TAGACACC929079409.380685781702605No Hit
ATTTGCTA746755937.5398106286213045No Hit
ACCCGACG735392927.425081116435001No Hit
CATGCGAT697040597.037847090229957No Hit
TGATATTC600004716.058099718981601No Hit
GTGATCGA583945235.895951106389892No Hit
CTACATCC482384754.8705199637783005No Hit
TCCTCCAA454793084.591933670225316No Hit
GATGTGGT436224334.40444957671405No Hit
AGGAGATG402681894.0657798247083425No Hit
ATTGGCGT356532293.599818684518104Illumina PCR Primer Index 6 (100% over 8bp)
CGCCATCG352952593.5636753356366615No Hit
TAGTTGTC345893973.492406188702136No Hit
GCAACAAA282404432.8513679467985495No Hit
GGGGGGGG214225892.1629860272389916No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)