FastQCFastQC Report
Tue 10 May 2022
HNTVLBGXL_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTVLBGXL_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10055438
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG105738110.515514092971385No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG8536208.489137917214546No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT8530538.4834991772611No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC7010836.972177641590551No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC6970656.932219163401933No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC5156515.128080944857897No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT4815504.788951013372068No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT4277034.25344972541226No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA2462682.449102664647726No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG2404642.391382652849135No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT2262652.2501754771895564No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA1860101.8498448302301698No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG1689311.6799964357594368No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC1351411.343959358110507No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC1302101.2949212157640473No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC1038051.0323269856569153No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT954690.9494265689868507No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC794220.7898412779234479No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG701370.6975031818604023No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA648710.6451335088536173No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT631240.627759825081712No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA460000.4574639115670545No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGTACCCCTACCAAC288310.28672047900847286No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGAACCCCTAC268660.2671788140904454No Hit
TAAGAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT207060.2059184294110311No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG203430.2023084424567085No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT168540.1676107992511117No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT105930.10534598293977845No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)