FastQCFastQC Report
Tue 10 May 2022
HNTVLBGXL_n01_THP1_Essen_Diff_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTVLBGXL_n01_THP1_Essen_Diff_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38141326
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC707098218.538899250644825No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA643498216.871416583681437No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA484810112.710887398094131No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC383345910.050670498450945No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC38138029.999133223632551No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC14426503.7823802979476904No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC12966213.3995173633973814No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA12603863.3045154224580444No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA9495052.489438883168351No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC7811842.048130156775357No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC7664932.0096128802653586No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGAACCCCTAC3917691.0271509700527979No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGTACCCCTACCAAC2510040.6580893385825128No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGTAACACCGAACCCCTACCAA2351590.6165464724535272No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGTTTTAAGACCTT1038940.2723922078639846No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC773410.20277480651826316No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCAA460980.1208610314177331No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGTTTTAAGACC384710.10086434855463598No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTTTTAAGAC384410.1007856937118547No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTTTTAAGA382240.10021675701573668No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)