FastQCFastQC Report
Tue 10 May 2022
HNTVLBGXL_n01_THP1_Essen_Diff_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTVLBGXL_n01_THP1_Essen_Diff_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45488773
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA760137416.710439738614184No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC603523813.267533067994602No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC598229513.151146108073744No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA566680512.457590359713594No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC528155211.610671494700462No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA14801473.253873213946659No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC13346102.933932731049923No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC12231972.6890085604199525No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC12162162.6736619165348774No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA11158832.453095404441883No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC10525372.3138390653007943No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGAACCCCTAC2683400.5899037989000054No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGTACCCCTACCAAC2617880.575500244862617No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGTAACACCGAACCCCTACCAA2156530.47407961520527275No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAGATAGTTCCCGT1630690.35848186100776996No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTAGCCAGAGATAC1284620.2824037482831203No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTTTTAAGAC1121470.2465377555908136No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGCACTGCTCATAA1076830.2367243451477577No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGTTTTAAGACCT1023860.225079713625162No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGTTTTAAGACC717090.15764109530938547No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC536960.11804231342973354No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTGTTCCTCCGCGG478310.10514902215542282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)