FastQCFastQC Report
Tue 10 May 2022
HNTVLBGXL_n01_K562_Essen_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTVLBGXL_n01_K562_Essen_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22240072
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG881990939.65773582027972No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG697149831.346562187388606No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG17703267.960073150842318No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG14251296.407933391582545No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG6137032.759447001790282No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1805850.811980284955912No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG1448970.6515131785544579No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTG449850.20227002862220952No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGG393550.17695536237472612No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT393340.1768609382199842No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACTG275360.12381254880829522No Hit
ATACACGATCTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT270520.12163629686090946No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAACTG266520.11983774153249145No Hit
GATCGCGCGGTTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG265980.11959493656315502No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTG262580.1180661645339997No Hit
GATCGCGCGGTTCTTGTGGAAGGACGAAACACCGAACCCCTACCAACTGG252230.11341240262171812No Hit
ATACACGATCTCTTGTGGAAGGACGAAACACCGAACCCCTACCAACTGGT235470.10587645579564671No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAACTGG228460.10272448758259416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)