FastQCFastQC Report
Tue 10 May 2022
HNTVLBGXL_n01_K562_Essen_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTVLBGXL_n01_K562_Essen_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40289544
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1450742236.00790815602182No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT807654420.046253191646947No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG685146517.005566009880877No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG29037277.2071478396479245No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT16656554.134211595941617No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG13748313.412376670234838No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG5802061.4400907590316734No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG1336720.3317783889537196No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTG781190.1938939790432972No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACTG499080.12387333050977195No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT490030.12162709014527442No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAACTG453380.11253043717744733No Hit
GATCGCGCGGTTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG421540.10462764234809906No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTG420220.10430001391924415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)