FastQCFastQC Report
Tue 10 May 2022
HNTVLBGXL_n01_K562_Diff_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTVLBGXL_n01_K562_Diff_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52882410
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1160197621.939196795304905No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT863089316.320914648178857No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG830889115.712012746771563No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC686308812.978016697801783No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT23653054.472763249632534No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT17896623.3842292739684146No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG16464753.1134643825801436No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC15909083.0083878552433596No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT14952652.8275280948806985No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC13934732.635040649622436No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG6961631.3164358432227277No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT3288440.621840040951235No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGTACCCCTACCAAC3120360.5900563155120956No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG1596620.3019189178405447No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT697670.13192855620611843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)