FastQCFastQC Report
Tue 10 May 2022
HNTVLBGXL_n01_GD_eRNA_052022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTVLBGXL_n01_GD_eRNA_052022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10422881
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT184463817.697966617866978No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG179988517.26859397128299No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA156742015.038260534683262No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT7569437.262320274020206No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC6688256.416891836335846No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA6407466.147494152528461No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC5760355.526638939847821No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG5496815.2737913826321146No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTGG1816861.743145681122139No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGTACCCCTACCAAC1555481.492370487583999No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT1446881.3881766471285628No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGTAACACCGTACCCCTACCAA521190.5000440856995297No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGCCCCTTGACCTT330580.3171675854305542No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA281210.26980064341135623No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTTATAGAACCTCC205710.1973638574593723No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG140420.13472282759440504No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC138020.1324202012859976No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT112340.10778209978603805No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)