FastQCFastQC Report
Tue 10 May 2022
HNTVLBGXL_n01_A375_Essen_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTVLBGXL_n01_A375_Essen_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26769628
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1272065047.51896440249375No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC623801223.302572602054834No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC402797115.046794822849238No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC7255092.710194553319904No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGAACCCCTAC2846581.0633618068954862No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT2422910.9050966266695973No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC1838930.6869464155422705No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT821080.3067207359026431No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT405020.15129832958455755No Hit
TAACAATGGTCTTGTGGAAGGACGAAACACCGAACCCCTACCAACTGGTC351510.1313092583879014No Hit
TAACAATGGTCTTGTGGCAAGGACGAAACACCGAACCCCTACCAACTGGT280420.10475304326231205No Hit
TAACAATGGTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC280340.10472315864830098No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAGGACGAAACACCGAACCCCTACC269960.10084562998036431No Hit
TACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC269630.10072235594756865No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)