FastQCFastQC Report
Tue 10 May 2022
HNTVLBGXL_n01_A375_Essen_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTVLBGXL_n01_A375_Essen_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26854536
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1037675838.640615499742765No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC654552224.373990300930913No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC491825718.314436711920845No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC10408273.875795880442693No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC9636973.58858183213443No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGAACCCCTAC3119441.1616063669839614No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT2462150.9168469714017774No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT678090.2525048282346044No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGTCGAAACACCGTACCCCTAC489210.1821703417255096No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT333630.12423599499168408No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAGGACGAAACACCGAACCCCTACC290830.10829827780304974No Hit
TAACAATGGTCTTGTGGAAGGACGAAACACCGAACCCCTACCAACTGGTC287780.10716252926507462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)