FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33775564
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA1729200.053.236994
TCGGAAG1759600.052.5140273
ATCGGAA1798300.052.0735472
GATCGGA1831200.051.2141761
GAGCACA1726050.050.8004269
AGAGCAC1769000.050.0065278
GAAGAGC1844150.048.7638786
AAGAGCA1958050.045.988147
GGAAGAG2106050.043.193115
CGTCTGA1303650.032.0129316-17
CACGTCT1341750.031.49521414-15
ACGTCTG1319750.031.44010514-15
ACACGTC1354600.031.3153912-13
CACACGT1428000.030.12239812-13
GCACACG1468250.029.2837210-11
GAACTCC1368950.029.17809120-21
TCTGAAC1475750.028.10628718-19
CTGAACT1459700.027.97679118-19
GTCTGAA1485500.027.92008216-17
TGAACTC1514200.027.02115620-21
Produced by FastQC (version 0.11.5)