FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb350.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb350.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40940256
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC11340942.770119463835302TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTATGC1093560.26711117780992866TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA2052900.066.162784
ATCGGAA2086550.065.5487442
TCGGAAG2083150.065.311533
GATCGGA2124950.064.395541
GAGCACA2129500.062.951629
AGAGCAC2170300.061.936698
GAAGAGC2255550.059.862196
AAGAGCA2365350.057.088987
GGAAGAG2611450.051.9497075
CTCGTAT1301700.044.7565442-43
CGTATGC1419700.044.5528444-45
TATGCCG1423650.044.39586346-47
TCGTATG1276950.043.9799142-43
ATCTCGT1353050.043.63898540-41
CCGTCTT1409000.043.62364650-51
TCTCGTA1313850.043.60516740-41
TGCCGTC1437550.043.6014848-49
ATGCCGT1410000.043.31478546-47
ACGGTAG1555900.043.13135532-33
GCCGTCT1397100.042.52819448-49
Produced by FastQC (version 0.11.5)