FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb349.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb349.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38688956
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC17885384.622864468092652TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATGC1957760.5060255438270291TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3015800.070.560394
TCGGAAG3059250.069.729023
GAGCACA3006400.069.615819
ATCGGAA3083050.069.448352
AGAGCAC3046300.068.863038
GATCGGA3137450.068.292681
GAAGAGC3153100.066.9916466
AAGAGCA3245950.064.957877
GGAAGAG3506050.060.527315
CTCGTAT2044100.045.2901442-43
CGTATGC2279050.045.02065344-45
TATGCCG2281050.044.9957546-47
ATCTCGT2084150.044.58169640-41
CCGTCTT2270800.044.54629550-51
TGCCGTC2297450.044.53092648-49
TCGTATG2027300.044.2397642-43
TCTCGTA2054550.044.00330740-41
ACGAGTG2399950.043.8121332-33
ATGCCGT2278400.043.73053446-47
TCACGAG2423500.043.4481330-31
Produced by FastQC (version 0.11.5)