FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb348.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb348.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40127692
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC18814644.6886922876102615TruSeq Adapter, Index 22 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTATGC1648010.41069144968517TruSeq Adapter, Index 22 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3032750.072.399614
ATCGGAA3048900.072.313512
TCGGAAG3051550.072.031683
GATCGGA3081850.071.5573351
GAGCACA3120200.069.7359
AGAGCAC3151550.069.176958
GAAGAGC3233550.067.693276
AAGAGCA3336250.065.571957
GGAAGAG3576600.061.3973245
CTCGTAT2127550.045.03062442-43
CGTATGC2295550.044.805744-45
TATGCCG2296750.044.72030346-47
TCGTATG2092600.044.6841142-43
TACGATC2420400.044.4863836-37
TCTCGTA2141750.044.46926540-41
CGTACGA2423950.044.4216734-35
CCGTCTT2262300.044.41145750-51
ATCTCGT2180800.044.35362240-41
TGCCGTC2304350.044.2554848-49
ATGCCGT2282200.044.20620346-47
Produced by FastQC (version 0.11.5)