FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb347.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb347.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33609946
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC14968094.453470410217261TruSeq Adapter, Index 21 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCGCGTATGC1522170.45289272407637904TruSeq Adapter, Index 21 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA2594050.065.5924764
ATCGGAA2634350.064.7751852
TCGGAAG2629200.064.7463763
GAGCACA2620950.064.23239
GATCGGA2676100.063.817931
AGAGCAC2652150.063.566228
GAAGAGC2730650.061.9941836
AAGAGCA2809350.060.1962787
GGAAGAG3023550.056.1649935
CTCGTAT1681500.044.98044642-43
CGTATGC1857150.044.89121644-45
TATGCCG1863750.044.79723746-47
TCGTATG1670250.044.55539742-43
CCGTCTT1852750.044.5068850-51
TGCCGTC1871450.044.4530248-49
ATCTCGT1710500.044.28038440-41
ATGCCGT1854500.044.22668546-47
TCTCGTA1687850.044.2118740-41
TTCGATC1904800.043.864336-37
CGATCTC1728200.043.80079338-39
Produced by FastQC (version 0.11.5)