FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb346.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb346.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35031314
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC9774582.7902407543148393TruSeq Adapter, Index 20 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATGC1030930.2942881331827861TruSeq Adapter, Index 20 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA1897600.058.8191344
TCGGAAG1933000.057.8209153
ATCGGAA1955750.057.2727362
GAGCACA1943300.056.9138879
GATCGGA2002150.055.940761
AGAGCAC1990800.055.6895458
GAAGAGC2061800.054.0145726
AAGAGCA2183450.051.0048077
GGAAGAG2373450.047.146645
CTCGTAT1105850.044.5093742-43
CGTATGC1231100.044.50099644-45
TATGCCG1239750.044.29401446-47
TGCCGTC1241100.044.1309948-49
CCGTCTT1239350.043.9863150-51
TCGTATG1098850.043.91974342-43
TCTCGTA1108300.043.5880540-41
ATCTCGT1130050.043.53512640-41
ATGCCGT1230600.043.5348946-47
GCCGTCT1240300.042.9933348-49
CGTCTTC1251000.042.5401450-51
Produced by FastQC (version 0.11.5)