FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb345.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb345.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43795199
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC13022572.9735154303100666TruSeq Adapter, Index 19 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTATGC1172260.2676686090637469TruSeq Adapter, Index 19 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA2482150.060.6980444
TCGGAAG2535600.059.476753
GAGCACA2509750.059.443619
ATCGGAA2576100.058.6840632
AGAGCAC2575300.058.1113248
GATCGGA2641750.057.2760961
GAAGAGC2670550.056.21636
AAGAGCA2802900.053.5700577
GGAAGAG3079650.048.9648675
CTCGTAT1494150.044.39922742-43
TCGTATG1484300.044.3818642-43
CGTATGC1634650.044.23698444-45
TCTCGTA1504450.044.07793840-41
ATGCCGT1637000.043.99648346-47
TATGCCG1648500.043.93879746-47
TGCCGTC1647750.043.79160748-49
CCGTCTT1635700.043.71638550-51
GCCGTCT1631600.043.6559348-49
AATCTCG1526600.043.5784138-39
ATCTCGT1530800.043.46815540-41
Produced by FastQC (version 0.11.5)