FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb344.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb344.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41898010
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC10492532.5043027103196547TruSeq Adapter, Index 18 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTATGC1083250.2585444988914748TruSeq Adapter, Index 18 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA1887600.063.934654
TCGGAAG1912600.063.2082983
ATCGGAA1918700.063.2031332
GATCGGA1953150.062.083621
GAGCACA2018800.059.1992349
AGAGCAC2050550.058.449398
GAAGAGC2117600.056.8800246
AAGAGCA2268200.053.153417
GGAAGAG2475300.048.8297545
CTCGTAT1190650.044.64443242-43
CGTATGC1320500.044.39986444-45
TCGTATG1184200.044.24181742-43
TATGCCG1328400.044.18227846-47
TCTCGTA1195450.044.0301540-41
TGCCGTC1335050.043.8519648-49
ATGCCGT1322250.043.7501546-47
CCGTCTT1329750.043.70526550-51
ATCTCGT1222600.043.5826940-41
ACGTCCG1370350.043.41340632-33
GCCGTCT1329400.043.13973648-49
Produced by FastQC (version 0.11.5)