FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb343.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb343.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43951119
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC12503512.844867271752512TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTCGTATGC7603511.7299923581012806TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATGC1238340.28175391848385023TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCGCGTATGC516020.11740770468210376TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3226550.073.297094
ATCGGAA3260200.072.995272
TCGGAAG3251250.072.915643
GATCGGA3295600.072.21841
GAGCACA3351450.069.7774669
AGAGCAC3380850.069.2999348
GAAGAGC3440350.068.418666
AAGAGCA3593300.065.4664467
GGAAGAG3823050.061.793655
CTCGTAT2248500.045.34726742-43
CGTATGC2444450.045.28656844-45
TATGCCG2519750.045.12364246-47
TGCCGTC2512700.045.0168448-49
CCGTCTT2480300.044.98346350-51
ATCTCGT2294200.044.70897340-41
TCGTATG2227900.044.61422342-43
ACGTCGA956250.044.50461232-33
TCTCGTA2259600.044.4339740-41
ATGCCGT2504550.044.22071546-47
GCCGTCT2474300.043.93017248-49
Produced by FastQC (version 0.11.5)