FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42506302
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC1023890.24087957592735307TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT268800.022.1590450-51
TATGCCG273450.021.92984846-47
ATGCCGT267600.021.68150746-47
TGCCGTC277750.021.62456748-49
TCGTATG240700.021.39123542-43
CGTATGC281250.021.2709844-45
CTCGTAT251600.021.16301742-43
GCCGTCT281950.020.49380948-49
CGTCTTC284800.020.27204950-51
TCTCGTA262650.019.5854840-41
ATCTCGT289150.018.4393840-41
GTATGCC358300.016.39189544-45
GCTTGAA368300.016.04355258-59
CATCTCG325400.015.83052238-39
TGCTTGA421800.014.44217858-59
AAGGGGG430600.014.0863666-67
CGTACGA11450.013.48238234-35
AGGGGGG365850.013.46348866-67
CGTCCAT437600.013.04173134-35
CCGTCCA474250.012.40444934-35
Produced by FastQC (version 0.11.5)