FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42263555
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC1877890.4443284527295444TruSeq Adapter, Index 15 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT373100.027.89327850-51
ATGCCGT379900.027.67528746-47
CGTCTTC381700.027.2274950-51
TGCCGTC388900.027.01653348-49
TATGCCG392400.026.89657446-47
GCCGTCT388700.026.68216948-49
TCGTATG363850.026.12201142-43
CGTATGC410000.025.70144544-45
CTCGTAT383850.024.92182542-43
TCTCGTA402250.023.9117440-41
ATCTCGT435400.022.16208340-41
GCTTGAA462300.022.1619658-59
GTATGCC480600.022.05933244-45
AATCTCG441100.021.80033538-39
AAGGGGG528450.020.2866466-67
TGCTTGA506550.019.80871658-59
AGGGGGG461850.017.72526766-67
AAAAGGG607500.017.56868464-65
GTCAATC642250.016.79559736-37
CGCGTAT64600.016.32309242-43
Produced by FastQC (version 0.11.5)