FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40508594
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC1517330.37456989990815287TruSeq Adapter, Index 14 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT321600.027.04304350-51
ATGCCGT326550.026.32762346-47
TGCCGTC334500.026.20602848-49
TATGCCG338200.025.95440746-47
GCCGTCT336350.025.58174748-49
CGTCTTC334150.025.52984250-51
TCGTATG306600.025.08172642-43
CGTATGC350600.025.02966144-45
CTCGTAT317300.024.70746442-43
TCTCGTA331000.023.2471540-41
ATCTCGT361400.021.77138140-41
GTATGCC415350.020.89328844-45
GCTTGAA418000.020.7040458-59
CATCTCG394000.019.602338-39
AAGGGGG467500.018.61343866-67
TGCTTGA476350.018.42717758-59
AGGGGGG403200.017.58822666-67
AAAAGGG545450.016.00127664-65
CGCGTAT62200.015.616569542-43
TCGCGTA62350.015.00764440-41
Produced by FastQC (version 0.11.5)