FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb339.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb339.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43080772
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG91900.024.16277942-43
CTCGTAT122700.022.878342-43
TCTCGTA129400.022.59303540-41
AATCTCG297600.021.61856738-39
CGTATGC93450.021.29685644-45
ATCTCGT208250.020.66445540-41
ATGCCGT93600.020.6537746-47
TATGCCG98800.020.09556246-47
TGCCGTC125550.019.52157248-49
CCGTCTT133800.018.88588350-51
GCCGTCT124600.016.90664348-49
CGCGTAT20000.016.74325442-43
GCGTGAA85850.016.68122158-59
CGTCTTC147900.016.57155850-51
TCGCGTA21650.016.12539140-41
AAGGGGG463400.015.63135466-67
ATCTCGG150550.014.71809940-41
TGCGTGA90600.014.05035858-59
ATCGCGT42150.013.86080140-41
CCGTCGT38900.013.79782650-51
Produced by FastQC (version 0.11.5)