FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb338.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb338.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44301169
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC973700.21979103982560821TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT297350.018.96113850-51
ATGCCGT296700.018.4183746-47
TATGCCG308400.018.22787346-47
TGCCGTC313100.018.09837248-49
TCGTATG277550.017.98640342-43
CTCGTAT292100.017.56204442-43
CGTCTTC313950.017.47443450-51
CGTATGC327750.017.115544-45
GCCGTCT324550.016.9256848-49
TCTCGTA309100.016.16585440-41
GCTTGAA368250.015.31697558-59
ATCTCGT343700.014.98068340-41
TGCTTGA407200.014.22512758-59
GTATGCC391150.014.17737844-45
AAGGGGG440150.012.94978866-67
CATCTCG387250.012.89734238-39
AGGGGGG382150.012.11239766-67
AAAAGGG500600.011.442945564-65
GTCTTCT607350.09.71324152-53
CTGCTTG611100.09.46321656-57
Produced by FastQC (version 0.11.5)