FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb337.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb337.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46108896
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1628220.35312491541762353TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT349950.025.65295446-47
CCGTCTT358050.025.50381750-51
TATGCCG361000.025.42034546-47
TCGTATG324750.024.68907742-43
TGCCGTC370900.024.68425848-49
CGTATGC376250.024.2953444-45
CTCGTAT336000.024.159342-43
CGTCTTC376650.023.79037750-51
GCCGTCT383000.023.5137648-49
TCTCGTA354100.022.66283840-41
ATCTCGT390300.020.9198940-41
TATCTCG388400.020.7531838-39
GTATGCC445900.020.38853544-45
GCTTGAA449650.020.14453358-59
TGCTTGA492850.018.5137258-59
AAGGGGG525400.017.16340666-67
AGGGGGG467350.015.82447666-67
GCTTATC582250.015.69558836-37
AAAAGGG586450.015.44142464-65
CGCGTAT68750.015.16503242-43
Produced by FastQC (version 0.11.5)