FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb336.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb336.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34760589
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3807431.0953295411651398TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC360110.10359720889654661TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT610050.035.9948850-51
TATGCCG630050.035.1613246-47
TGCCGTC629600.035.15255448-49
CGTATGC651800.034.00987644-45
ATGCCGT617850.033.856846-47
CTCGTAT593000.033.83788342-43
CGTCTTC622150.033.28310450-51
GCCGTCT629200.032.98944548-49
TCGTATG581050.032.78851342-43
ATCTCGT639850.031.46054640-41
GCTTGAA655100.031.34072358-59
TCTCGTA610800.031.2848840-41
TGCTTGA683150.031.26715958-59
AAGGGGG709950.030.58874366-67
GTATGCC700300.030.02665944-45
GATCTCG665350.028.8627138-39
AAAAGGG755350.028.53635664-65
GTCTTCT845400.025.66247752-53
ACGATCA892400.025.1259232-33
CGCGTAT88550.024.86243842-43
Produced by FastQC (version 0.11.5)