FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb335.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb335.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34942526
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC569160.1628846180153089TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT133700.026.62699346-47
TCGTATG139850.025.99947742-43
TCTCGTA183450.022.44825740-41
CGTCTTC173700.022.32740650-51
GCCGTCT167800.022.00849348-49
TATGCCG142450.021.19019746-47
CGTATGC146050.020.8467544-45
CCGTCTT165000.020.3812750-51
CTCGTAT173100.020.20959342-43
TGCCGTC171750.020.02274948-49
GTATGCC206100.018.6791444-45
AATCTCG301650.018.58848238-39
ATCTCGT251800.017.75068740-41
AAGGGGG392950.016.75965566-67
GCGTGAA76200.016.26924758-59
TCGCGTA33600.015.76220140-41
GCTTGAA245350.014.47128258-59
TGCGTGA83150.013.96685158-59
AAAAGGG416800.013.5727164-65
AGGGGGG354450.013.48780666-67
Produced by FastQC (version 0.11.5)