FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb334.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb334.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34885800
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC11935733.421372019560968TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC1142290.3274369514243618TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA2016450.066.919114
TCGGAAG2049100.065.8690643
ATCGGAA2059700.065.664532
GAGCACA2051900.065.237469
GATCGGA2097650.064.456151
AGAGCAC2085600.064.338198
GAAGAGC2168400.062.1177866
AAGAGCA2262400.059.5720447
GGAAGAG2474550.054.642075
CTCGTAT1333300.045.33092542-43
CGTATGC1467350.045.1793344-45
TATGCCG1476750.044.99145546-47
TCGTATG1328550.044.86017642-43
TCTCGTA1339600.044.55051440-41
ATCTCGT1358150.044.52433440-41
TGCCGTC1490900.044.4641548-49
CCGTCTT1488150.044.37396250-51
ATGCCGT1474200.044.3314446-47
GCCGTCT1490100.043.67836848-49
CATCTCG1372800.043.38659738-39
Produced by FastQC (version 0.11.5)