FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46369092
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC16254273.5054104574659344TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGC1372080.29590400433116093TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATGC649580.1400890058403559TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATTC567780.12244794441952842TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3022600.067.288114
TCGGAAG3071250.066.32133
ATCGGAA3085950.066.199472
GAGCACA3080150.065.483359
GATCGGA3140700.065.013661
AGAGCAC3125350.064.660938
GAAGAGC3236250.062.679646
AAGAGCA3351400.060.5340927
GGAAGAG3644350.055.861765
CTCGTAT1876950.045.00860242-43
CGTATGC1961950.044.84530644-45
TATGCCG1960250.044.70125246-47
TCGTATG1804250.044.5489142-43
CCGTCTT1926450.044.2355350-51
TCTCGTA1896000.044.23192640-41
ATCTCGT1968600.044.20401440-41
TGCCGTC1974850.044.15315648-49
CGATCTC2063400.044.11022638-39
ATGCCGT1924200.044.099646-47
CACGATC2276800.043.91774736-37
Produced by FastQC (version 0.11.5)