FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb332.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb332.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37571949
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC30688388.16789674658613TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC2941290.7828420080097521TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4050000.087.382242
TCGGAAG4063250.086.980693
GATCGGA4071900.086.971951
CGGAAGA4067100.086.829334
GAGCACA4188000.083.726079
AGAGCAC4218250.083.233768
GAAGAGC4248400.082.836346
AAGAGCA4370950.080.474177
GGAAGAG4529750.077.841395
TCGTATG3341550.046.30042642-43
TCTCGTA3384350.046.22783340-41
ATGCCGT3668500.046.0478946-47
AATCTCG3412700.046.0115238-39
GCCGTCT3622700.045.82435248-49
CTCGTAT3371450.045.7228642-43
CGTCTTC3625750.045.58930250-51
CGTATGC3686050.045.4678444-45
GTATGCC3749350.045.41344544-45
TATGCCG3691450.045.39746546-47
ATCTCGT3412450.045.37808640-41
Produced by FastQC (version 0.11.5)