FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34579699
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC17240244.985653576683823TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC1746330.5050159632679279TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2509600.079.021572
CGGAAGA2502250.078.899724
TCGGAAG2514250.078.65553
GATCGGA2529300.078.4251
GAGCACA2605350.074.975699
AGAGCAC2630350.074.412978
GAAGAGC2687400.073.2055056
AAGAGCA2785850.070.6180657
GGAAGAG2977950.066.281875
CTCGTAT1913750.045.60021242-43
CGTATGC2126300.045.3833844-45
TATGCCG2142350.045.19746846-47
ATCTCGT1937850.045.04042440-41
TCGTATG1908200.044.9275842-43
TGCCGTC2151550.044.86840448-49
CCGTCTT2143000.044.8368250-51
TCTCGTA1919200.044.74181740-41
ATGCCGT2137900.044.4306246-47
GCCGTCT2145850.044.04351848-49
CATCTCG1952800.043.88445738-39
Produced by FastQC (version 0.11.5)