FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48376021
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC21279534.398776410321139TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC1854550.3833614178396359TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3600850.066.196594
TCGGAAG3655350.065.308583
GAGCACA3643100.064.8705449
ATCGGAA3694800.064.771782
AGAGCAC3698050.064.051768
GATCGGA3749900.063.8162461
GAAGAGC3811800.062.3596466
AAGAGCA3955300.060.1137777
GGAAGAG4256050.056.041635
CTCGTAT2379500.045.1791342-43
CGTATGC2605900.045.05073544-45
TGCCGTC2621800.044.83640748-49
TATGCCG2627050.044.7901946-47
CCGTCTT2616700.044.78038850-51
ATCTCGT2410750.044.5846440-41
TCGTATG2373500.044.49585742-43
TCTCGTA2383600.044.318340-41
ATGCCGT2616200.044.09267446-47
TATCTCG2390600.044.0534438-39
GCCGTCT2622600.043.87369548-49
Produced by FastQC (version 0.11.5)