FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42340729
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC19434494.59002252889883TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC1800720.4252926301764904TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3215400.069.033444
TCGGAAG3257750.068.2849043
ATCGGAA3282950.067.966742
GATCGGA3320450.067.19781
GAGCACA3289550.066.757249
AGAGCAC3328700.066.107648
GAAGAGC3405400.064.934546
AAGAGCA3552350.062.234887
GGAAGAG3783000.058.6794555
CTCGTAT2194200.045.05371542-43
CGTATGC2424500.044.84029444-45
TATGCCG2434350.044.73690446-47
TGCCGTC2434350.044.69690748-49
CCGTCTT2423650.044.64825450-51
ATCTCGT2227500.044.4196640-41
TCGTATG2187450.044.33719642-43
TCTCGTA2199450.044.13419340-41
ATGCCGT2426100.043.97570846-47
GCCGTCT2427750.043.75848-49
GATCTCG2224400.043.54627638-39
Produced by FastQC (version 0.11.5)