FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36539850
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC22120226.0537249058219995TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC3415480.9347274277261675TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGC2325250.6363600288452197TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGC2149270.588198911599254TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC2001980.5478894959886261TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGG844900.231227002847576TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATTC490790.13431636966216337TruSeq Adapter, Index 4 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGC421240.115282356112573TruSeq Adapter, Index 4 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA4666050.082.902544
ATCGGAA4711300.082.631362
TCGGAAG4692950.082.603583
GATCGGA4731900.082.2898251
GAGCACA4724650.080.894659
AGAGCAC4766000.080.3871468
GAAGAGC4817450.079.820686
AAGAGCA4950800.077.698217
GGAAGAG5108150.075.523865
TCGTATG2523900.045.64969342-43
CTCGTAT2763450.045.58652542-43
TCTCGTA2784850.045.5764940-41
ATGCCGT2530500.045.4658746-47
ATCTCGT3182800.045.2489840-41
CGTATGC2679350.045.2313444-45
GCCGTCT2481100.045.1372748-49
CGTCTTC2532350.045.12879650-51
TATGCCG2666350.045.0893546-47
TGCCGTC2767000.045.08858548-49
CCGTCTT2667450.045.02000450-51
Produced by FastQC (version 0.11.5)