FastQCFastQC Report
Wed 2 Sep 2020
HNTFYDRXX_n01_mb327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFYDRXX_n01_mb327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44735091
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC22265094.977097285886822TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC2330750.5210115700893512TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3890250.065.009934
GAGCACA3874050.064.853219
TCGGAAG3939500.064.274593
AGAGCAC3941250.063.8089078
ATCGGAA3990000.063.5609932
GATCGGA4057650.062.57161
GAAGAGC4058750.062.149116
AAGAGCA4187900.060.2265627
GGAAGAG4469500.056.6111765
TCGTATG2465850.045.19979542-43
TCTCGTA2471850.045.07753840-41
CTCGTAT2470700.045.06685342-43
ATGCCGT2763150.044.9958746-47
CGTATGC2757500.044.88738644-45
GCCGTCT2765850.044.8076648-49
CCGTCTT2755800.044.7728350-51
TGCCGTC2770350.044.77002748-49
TATCTCG2480250.044.7640138-39
TATGCCG2772100.044.76320646-47
CGTCTTC2773750.044.5575850-51
Produced by FastQC (version 0.11.5)