FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MTH-037_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-037_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28638950
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG2114400.7382952238123255No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA1750010.611059413840242No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA1347270.4704327498040256No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1214930.4242229551013567No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG1145600.4000146653421302No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC1076370.3758412930641661No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA1002870.3501769443362972No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA968770.3382700832258166No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC944490.3297921187753043No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA887920.31003929962516086No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA782120.27309660444953465No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA769100.2685503483891693No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT761870.26602581449389734No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA706970.24685611728083606No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA696240.24310947154137985No Hit
GGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATT677590.236597361285941No Hit
CAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTT675400.2358326684462943No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT669240.23368175160053004No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT656320.2291704130214271No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA651180.22737565448453942No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC647500.22609069117408287No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA644060.22488952981865604No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA639110.22316111449616696No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT613290.21414542083421353No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA605000.21125076163756004No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT604970.2112402863931813No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT599590.20936172590126384No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG597180.20852021460283984No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA595370.2078882081919903No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC584940.20424631489632128No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT580640.20274486320203777No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC572460.19988861323477292No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA569290.19878172907875463No Hit
GTTCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC562090.19626767042786136No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG556970.19447989538722613No Hit
CATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAAC544930.1902758306432324No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT543370.18973111793553885No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA526980.18400814275663038No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT517870.18082716021362513No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT513960.17946188669626506No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT508400.17752047473807525No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC500020.17459438980828557No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG499420.1743848849207111No Hit
AGTAATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAA489690.17098741399387896No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA489220.17082330183194566No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG486460.16985957934910323No Hit
GAGCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAG485520.1695313550252366No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG479380.16738742167572485No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT468180.1634766637743353No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACT467530.16324970014612966No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG460860.16092070414592713No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT457180.15963574083547058No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT455930.1591992723196905No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG448680.156667754928166No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT440980.1539791088709607No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG430770.15041403403406897No Hit
CAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCA430660.15037562480468034No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG427810.14938047658870174No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT424970.1483888201208494No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA424490.14822121621078987No Hit
GACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTC418680.14619251054944402No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA415060.1449284977277449No Hit
GCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAA404840.14135993114272696No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA402950.14069999074686745No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT395500.13809863839281816No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG392480.13704413045869349No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG390550.13637022307032906No Hit
CTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAAAAGTAAAGCGGG389750.13609088322022977No Hit
CTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGT379260.13242803943580334No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA377980.13198109567564453No Hit
ATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAACAGTT376620.1315062179304758No Hit
GAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGT375130.13098594745966596No Hit
GAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCATAATTAATG370730.12944957828412007No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC367520.1283287271355968No Hit
ACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTT365070.12747324884466785No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA364330.12721485948332603No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA359610.12556675436774042No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC357900.12496966543815329No Hit
CTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAATTATCCG357890.12496617369002704No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAG354630.12382786380087259No Hit
GAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCC351760.12282573208864153No Hit
CTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACG350600.1224206893059976No Hit
GATTCTTCGGATTTATTTTTCTTCTTTATTAAAAGGAACATCAGCGAACT345770.12073417496102337No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT342900.11973204324879229No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT341490.11923970676299235No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG335970.1173122617973075No Hit
CTGAATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAAC331320.11568859891860561No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC328640.11475281042077311No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC320730.11199083765291674No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA320520.1119175109422657No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTTGGAA315030.11000054122095956No Hit
GAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAG308760.10781121514580667No Hit
GAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGG303070.10582441046197574No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT302350.10557300459688641No Hit
AGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCC302320.10556252935250768No Hit
GTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAA302300.10555554585625519No Hit
CCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAA299390.10453944715151918No Hit
GTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAACTTCCTGTCGGC298670.10428804128642985No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT292930.1022837778619677No Hit
AGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCA292220.10203586374500462No Hit
GATATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATG291720.10186127633869259No Hit
ATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAACAAG289800.10119086069845437No Hit
AGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGA289000.10091152084835513No Hit
ACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATAC288410.10070550770890693No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCATAATTAATGTAATA286780.1001363527643297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)