FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MTH-034_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-034_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26453943
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG1984210.7500620984932189No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA1464470.5535923321525263No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA1025880.3877985221333546No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA988230.37356623925590227No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA927970.35078702634234904No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG889370.33619562875749753No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC878880.33223024635684745No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC854860.32315031449186987No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA849410.32109013011784293No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG816580.30867988186108963No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA811090.3066045768678038No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC740730.28000740759137493No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA737840.2789149428499184No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA679140.25672543408746284No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC676700.25580307631266913No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG657960.2487190661898682No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA654290.24733174937286287No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT649880.24566470109956765No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC636740.24069757767301456No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC632400.23905699048342244No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA622420.23528439597832354No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA620030.23438093897760345No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT615080.23250976234431292No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT607290.22956502174363952No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT600480.22699073631480948No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT599780.22672612547777848No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG579470.21904863104906516No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG566990.21433099784028414No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT565610.21380933647585162No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC538770.2036634009531207No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT526350.19896844867322802No Hit
GACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTC499230.1887166688156847No Hit
CAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTT493480.18654307979721585No Hit
GGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATT493220.18644479577203293No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA471010.1780490719285212No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACT470900.17800749022555917No Hit
GAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCC464350.1755314888219121No Hit
ACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTT461650.17451084702193545No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA446050.16861380551095917No Hit
ATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAACAGTT445880.16854954287910878No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT441130.1667539693421128No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT440120.16637217370582524No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT436730.16509070122363234No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA433340.1638092287414394No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC432390.16345011403404022No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT430080.16257689827183797No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT429220.16225180495777133No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT424330.16040330925336915No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC424180.16034660693114822No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC420100.15880430376673904No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT418030.15802181172009025No Hit
GAGCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAG415580.15709567379048184No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT397080.15010238738323434No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA381060.14404657937003945No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT380760.1439331747255976No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG379130.1433170094907969No Hit
GAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAG376650.14237953109674426No Hit
CTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAAAAGTAAAGCGGG373660.14124926480714048No Hit
AGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCA373500.14118878233010482No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG369080.13951795390199487No Hit
GAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATG367520.13892824975089726No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCA366910.13869766030719882No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA362340.13697012955686794No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG359610.1359381472924471No Hit
CTGAATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAAC358610.13556013181097426No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA354800.13411989282656275No Hit
GATTCTTCGGATTTATTTTTCTTCTTTATTAAAAGGAACATCAGCGAACT354070.13384394152508758No Hit
CATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAAC352020.13306900978806827No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTT349670.1321806734066071No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT342840.12959882766814762No Hit
CAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCT342530.12948164286889105No Hit
GCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAA340910.12886925778890504No Hit
GGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTG339420.12830601472151051No Hit
GTTTCTTTTTCGCGTAAGAATGTATTTGCTTGATTGTAAAGCTTCTTAGA339190.12821907116077175No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG327860.12393615575568451No Hit
AGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGA316900.11979310607874222No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG310410.1173397856039835No Hit
GAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACC301120.11382802178110084No Hit
GAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGAT288370.10900832439232216No Hit
CGGCGAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA287860.108815536496771No Hit
GTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTA284720.10762856788494629No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA280320.10596529976646582No Hit
CCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAA272960.10318310582282572No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT272010.10282399111542653No Hit
AACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTA271450.10261230244580175No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG271080.10247243671765678No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA270880.10239683362136223No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG270610.10229476944136456No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG269710.101954555508039No Hit
CAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCA267310.10104731835250419No Hit
GTTAAGATGGAACGGATTAAGAGAGAAAAAAAGTAAAGCGGGAAAATGAT265180.10024214537696706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)