FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MTH-033_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-033_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19633483
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA1462930.7451199565558491No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA921670.46943784757905666No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA901600.4592155146389461No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG855300.43563335145373855No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG836060.42583376571543624No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA773600.3940207654444196No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG733720.37370852639849994No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA658770.33553394474123616No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT641300.32663588014413947No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT590710.30086867419295904No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA590640.30083302081449326No Hit
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATACAAGGCCA489550.24934444897015978No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA486980.24803546064648846No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT479220.2440830289765703No Hit
GGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGA476880.24289118746785784No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT466180.23744131390237788No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC448850.2286145560622127No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC442060.2251561783510343No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT436310.22222750797706142No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC436120.2221307345212258No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA430560.21929883760308855No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA427140.21755691539804728No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA393520.20043310705492248No Hit
CAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTT390480.19888473176155244No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG388570.19791190386341537No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT379590.19333808474023687No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC361660.18420572651322234No Hit
CGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTCATAGTGTTTC360360.1835435923417154No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT353180.17988657437908495No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT351340.17894939985941363No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA339450.17289341886001583No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC339060.17269477860856375No Hit
GGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATT329470.16781026575875507No Hit
AGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGATTTCT327450.16678141112302897No Hit
GAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAG323650.16484594200631644No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA323530.16478482192894658No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG323000.164514874920563No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA320130.16305308640346697No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCA315560.16072543012363116No Hit
GAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGT314850.16036380299919276No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT309800.15779166640987746No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA302580.1541142750881237No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT300330.15296827363743865No Hit
CATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAAC293980.14973400287661642No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG293830.1496576027799041No Hit
GAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGATTT293380.1494284024897671No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG287000.1461788517096024No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA286220.14578157120669827No Hit
GAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAG285530.1454301307618215No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG284580.1449462634826434No Hit
AGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATAC279680.14245052699004043No Hit
CTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAA278680.1419411930119582No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT277660.14142167235431433No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA275210.14017380410801283No Hit
CAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCA274230.13967465680949223No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA273950.1395320432956292No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT271680.1383758551653825No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT271320.13819249493327293No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG270050.13754564078110848No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG269740.13738774724790298No Hit
ATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAACAGTT269710.13737246722856053No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT268230.13661865294099879No Hit
AGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGA267400.13619590573919055No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG266560.13576806519760146No Hit
CTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGT266390.1356814784213275No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG266340.13565601172242336No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG265290.135121211045437No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG260920.13289542156121764No Hit
GTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAA254410.12957965736390226No Hit
ATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTA253560.12914672348253237No Hit
AGTAATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAA251580.12813824220592954No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA250180.1274251746366144No Hit
CTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACG247000.12580549258631288No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA244170.12436407742834016No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACT242490.12350839634516197No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC242470.12349820966560035No Hit
CTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAATTATCCG240210.12234711487513449No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC238690.12157292722844949No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC237180.12080383292154531No Hit
GTTGTTTTACTTATCTATTGATGGGGAAATATATTACTCGGTTCGCCGGG233770.11906700405628487No Hit
CTGGAGTTGACTAATATAATTTTACCAGGTAGAGATAATGATTAGAGGAA233260.11880724372746293No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC230700.11750334874357239No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT228530.11639809401113395No Hit
GTTTTACTTATCTATTGATGGGGAAATATATTACTCGGTTCGCCGGGTAT224500.11434547807946252No Hit
GGGAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATA220760.11244056900143494No Hit
GTGAAATACCACTACTTTTAAAGTTGTTTTACTTATCTATTGATGGGGAA215040.10952717864680456No Hit
GTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAACTTCCTGTCGGC214880.1094456852103114No Hit
CTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAG214540.10927251165776342No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA212740.1083557104972154No Hit
GTAAGAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGA212490.10822837700269483No Hit
GTTTCTTTTTCGCGTAAGAATGTATTTGCTTGATTGTAAAGCTTCTTAGA211890.1079227766158455No Hit
CTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAA211590.10776997642242081No Hit
GAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGATTTCTA209160.10653229485568097No Hit
CGGCGAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA207520.10569698713162612No Hit
GAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACC206010.10492789282472194No Hit
ATTGGCTCTGAGGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAAT205920.10488205276669453No Hit
GGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCGTAGTTTGTTGCTTTCTAT204170.1039907183050506No Hit
GAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTA202020.10289565025217379No Hit
CTGAATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAAC201770.10276831675765324No Hit
GTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTA201420.10259004986532445No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT200410.10207562254746139No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA200150.10194319571316002No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG199540.10163250198652984No Hit
GCTCATTAAAACAGTTATAATCTACTTGACATTTTTTCTATAAGGATAAC198620.10116391472669418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)