FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MTH-025_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-025_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33692085
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG2262210.6714366297010114No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA2194810.6514319312681303No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA1131770.33591569058430193No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1110160.32950172124996124No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA1044280.30994816735147146No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG1040070.3086986157134532No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC732160.21730919888157707No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT720350.2138039245716019No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC706860.20980001682887836No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA673970.20003808016037No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC669590.19873807156784748No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACAC667260.19804651448552382No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT645260.19151679096143798No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC644580.19131496314342078No Hit
GTTAAACATTTGGTACTTCGTTGCCTTCTATACGCACAATTAATGTAATA623410.18503158827956181No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA621830.18456263540828655No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA617110.1831617129067554No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT616000.1828322586744038No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT610850.18130370975853824No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC587200.17428425697014596No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA578950.17183561064861377No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT571140.16951755879756328No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG570080.16920294484594825No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA562580.16697690273546445No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT534590.15866931357913885No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA513120.15229689703086052No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT508250.1508514536871197No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG506500.15033204386134014No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT498080.14783294058530363No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA497890.14777654751850472No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT497460.1476489211041703No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC494840.14687129039357463No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG493490.14647060281368754No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG470720.13971233896625868No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA464290.13780387886353723No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA454090.13477646159327927No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC453920.13472600463877496No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA443290.13157096095418255No Hit
CAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTT441930.1311673053181482No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT438820.1302442398563342No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA435810.12935085495599336No Hit
GGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATT432790.12845450199950523No Hit
GAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCC426490.1265846266266988No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC423760.1257743472984827No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC422940.12553096669440314No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG422310.1253439791571225No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG407700.12100764912590005No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA407260.12087705465541833No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG399830.1186717889379657No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACACTTCTAGCTTAATCCA395560.11740442896306358No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT395500.11738662062617972No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA394600.11711949557292164No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA389880.1157185730713905No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC385670.11446902143337225No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT384090.11400006856209702No Hit
CTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTTGGTAC383460.11381308102481637No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG381320.11317791700929164No Hit
CATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAAC373330.11080644014758957No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT372570.11058086788039387No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG370560.1099842885947842No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA368710.10943519820753153No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA363600.10791852151625524No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT362050.10745847281342191No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC361920.10741988808350685No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG361890.10741098391506491No Hit
ATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTTGGTACGAGAG353940.10505137927795208No Hit
GAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATG343660.10200021755851559No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA338700.10052806170944897No Hit
CTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGT337010.1000264602205533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)