FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MTH-021_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-021_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27802123
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG1567720.5638849954012505No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1452340.5223845675382416No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA1040740.3743383194153914No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG881640.31711247374885726No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT870500.31310558549791323No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC783350.2817590584719016No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA777780.2797556143464296No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG716290.25763859831855285No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC696490.2505168400269289No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA666430.23970471607509974No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT649660.23367280261295154No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG641150.23061188528660204No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA640170.2302593942196429No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT619060.22266644888953263No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC578170.2079589389630425No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC569250.20475055088418967No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA556810.20027607244238144No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA537760.19342407772240988No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT532050.19137027773022947No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA522790.1880395968322275No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT517930.18629152888791983No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG516850.18590306934474032No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA510370.18357231208566338No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT477140.17161998743765No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA465880.1675699370152416No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT444290.15980434299927385No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG443430.15949501410377906No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT441590.1588331941413251No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT436420.1569736239207344No Hit
GAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAA434860.15641251569169734No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA422190.1518553097545824No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA409150.14716502045545227No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA405050.14569031293041904No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG404920.1456435539113326No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA394230.14179852380338004No Hit
CTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTT394050.14173378054618346No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA391910.14096405515506855No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT391280.14073745375488053No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG390050.14029504149737054No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC388750.13982745130650634No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG380600.13689602049455No Hit
GGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTT380570.13688522995168392No Hit
GAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGT380440.1368384709325975No Hit
GAGCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAG370340.13320565483434485No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA360550.1296843410123752No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG351080.1262781263143106No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT349980.12588247307588704No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT348490.12534654278020424No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA340700.12254459848264107No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG331680.11930024192756791No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT326060.11727881356398573No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA325230.11698027521135707No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC325060.11691912880178251No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG321920.11578971864846437No Hit
GCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAA320280.11519983563845107No Hit
CGGCGAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA316220.11373951550390596No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA313330.1127000265411386No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA306370.11019662059620411No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA305900.11002756875796858No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA301420.10841618102329811No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA299710.10780112007993058No Hit
GAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTA297540.10702060414594958No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT296190.10653502971697522No Hit
GCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTC293980.10574012639250607No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA293330.10550633129707399No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT289490.10412514181021355No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT289210.10402443007679665No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA288570.10379423182898659No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT283000.10179078770351459No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC282800.10171885075107394No Hit
GAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTTT278710.10024774007366272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)