FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MTH-019_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-019_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43856866
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAG1083190.24698299235517648No Hit
GTCTACTTGACATTTTTATTATAAGGATAACTACGGAAAAGCTGTAGCTA623940.14226734760299561No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA589760.13447381306270267No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA467230.10653520021243652No Hit
GGAGTCTAGCAAATGTGCAAGTGTATATGATTCTTTAAACATTTCTCTTT466900.1064599554377643No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGTTAAAGCATTTAC458240.10448535013879014No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA455760.10391987425640492No Hit
GTTGAACTCAATTCAAAAAATTTCTTGAACGGGGTTTTCGGATTCAGTTC455080.10376482441768638No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT447560.10205015561303445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)