FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MTH-006_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-006_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35322487
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG3423210.9691305145076563No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA1952750.5528347989766406No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1511150.4278152894500322No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT1294580.3665030721081446No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG1120000.31707846619067337No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC1073380.3038800750354866No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA1065560.30166618788761956No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC1009310.28574148813473976No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT914220.25882096014360484No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA796840.22559000446372873No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA775380.21951455456689672No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA761480.21557938431685178No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA751460.21274266446753876No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG750970.21260394263858035No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA745980.21119124482939156No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA744400.21074393770744398No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA724720.20517241608723644No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT710050.2010192543916854No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT690930.1956062720045732No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA690330.19543640854054245No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT682620.19325366302774774No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG660690.187045153417425No Hit
GAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAA631210.17869919521804906No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG612780.17348155581457217No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA605260.1713526003987205No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC600520.17001067903287784No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC565720.1601585981190962No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT562030.15911393781530728No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA545590.15445967890086562No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT536110.15177583616918028No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG523410.14818039284719672No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG513010.14523609280399766No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT510400.14449718673546402No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC508490.14395645470829957No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA506240.14331946671818438No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT503670.14259188488058613No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG502370.14222384737518623No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA494270.13993069061077154No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT487320.13796310548574905No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC483730.1369467557592986No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG471390.1334532305157335No Hit
GCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAA466450.13205468799521392No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG459460.13007577863925607No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA449220.12717677551979847No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT445090.1260075486757204No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA434640.12304909334385204No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA432880.12255082718269526No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT432500.12244324698880915No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA432420.12242059852693837No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA431130.12205539207927234No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC430350.12183456957603239No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG428780.12139009351181869No Hit
GAGCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAG424330.12013027282025752No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT415760.1177040563423521No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA399620.1131347291599258No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA395120.1118607531796954No Hit
TGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAA389920.11038860315809587No Hit
CTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTAATT387940.10982805372679448No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG387680.10975444622571451No Hit
AATGGATCCGTCCATTTTTAGTGTGTATCAATCGAGTTTCTGACCTATCA386400.10939207083578231No Hit
CTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAG384390.10882302823127941No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTT383520.10857672620843486No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG382760.10836156582066264No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG382000.10814640543289039No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA380650.10776421263882129No Hit
AGTAATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAA380380.10768777408000745No Hit
ATTTTTTTGGGGTTTCGGCTCTGAATTCTTAATTTCCTGTTTTGTAGTAA376200.10650439194726011No Hit
CTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAAAAGTAAAGCGGG370600.10491899961630674No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC367380.10400739902600856No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT366880.10386584613931629No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT363770.1029853871840904No Hit
AGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAAT360110.10194922005350303No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT357890.10132072523658937No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG355370.10060729868766037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)