FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MTH-005_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-005_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36856290
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG3355390.9103981979738058No Hit
CTCAAAAGGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCA2233370.605967122572565No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA2199970.5969048973730129No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA2106750.5716120640465983No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG2008100.544845940814987No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA1580940.4289471349395178No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA1526210.41409756652120977No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA1487870.40369500022926885No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC1411420.38295227219017436No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA1402940.3806514437562761No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT1399330.37967196372722267No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA1348910.3659918022134078No Hit
GGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1264230.3430160767673578No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC1180870.32039849914356544No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT1166400.3164724393041188No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA1137800.3087125698218676No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT1129950.30658267557586505No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT1114850.3024856815485226No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA1051330.2852511742229074No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT1031580.27989252309442975No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG997020.27051556192986326No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT986420.26763952638749045No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT976950.2650700870868989No Hit
CAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTT967110.262400257866432No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT960740.2606719232999306No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA950950.25801566028485234No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA937980.2544965866070622No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT922730.2503588939635541No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG878380.23832566978390932No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG870050.23606553996617674No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT865380.23479845638288607No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA810170.219818652392848No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA804680.2183290830411851No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT792700.21507862023008828No Hit
CATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAAC791490.21475031805968534No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG788040.21381424988787529No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA787580.21368944079830066No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG773050.2097471015123877No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA769810.20886801140320962No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT733200.19893483581771254No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG732950.1988670047907698No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC728080.1975456563859249No Hit
AAAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTT724320.19652547774070583No Hit
GGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATT713960.1937145599841981No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG702350.19056448709297652No Hit
CTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGT694840.18852684304361617No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA687860.18663300077137443No Hit
AAGGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTA665560.18058247316808068No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT661570.1794998899780743No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA661470.1794727575672972No Hit
CAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCA655890.1779587690459349No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA654960.17770643762570784No Hit
GAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTA652620.17707153921352364No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT634840.1722473965773549No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA634530.1721632861039459No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC631690.1713927256378762No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG630830.1711593869051931No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA621300.1685736681581353No Hit
ATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAACAGTT580200.15742224732874635No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC553850.15027285708897992No Hit
CTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAATTATCCG552470.14989842982025592No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT550020.14923368575621693No Hit
ACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATAC538410.14608361286499535No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAAATATGCATTTCAT524990.14244244333870826No Hit
CTGAATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAAC524360.14227150915081252No Hit
CAAAAGGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG520150.14112923465709654No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG511280.13872258982116756No Hit
CTGGAGTTGACTAATATAATTTTACCAGGTAGAGATAATGATTAGAGGAA509620.13827219180226766No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC500540.1358085689037068No Hit
GTTTCTTTTTCGCGTAAGAATGTATTTGCTTGATTGTAAAGCTTCTTAGA495280.13438140409683122No Hit
GGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTT494800.13425116852510113No Hit
CTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTAGAGATAATG493730.13396085172978617No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA491500.1333557989694568No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC486130.1318987885107264No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC485240.13165731005481018No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG479190.13001579920279552No Hit
CTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACG474850.12883825257506928No Hit
CTTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATG472820.12828746463629412No Hit
CGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTTATAGTGTTTC470850.12775295614398519No Hit
AGTAATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAA464330.12598392296131813No Hit
GTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAA463890.12586454035389888No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA462610.12551724549595197No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAAATATGCATTTCATCCCCGTCTC461800.12529747296865745No Hit
GTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAAAGA460840.12503700182519725No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT459880.12477653068173708No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA459160.12458117732414195No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA454990.1234497557947368No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT454680.12336564532132778No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG444170.12051402894865436No Hit
TCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAG444040.12047875681464412No Hit
CCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC441590.11981401275060512No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC439730.11930934991015102No Hit
AGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGATTTCT439450.11923337915997513No Hit
CGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGT438320.11892678291819388No Hit
GAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGT437990.11883724596262946No Hit
CTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAG436430.11841398035450665No Hit
TGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAA436380.1184004141491181No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG434930.11800699419285013No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA431110.11697053610116481No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA429820.11662052800214022No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG429590.11655812345735288No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGGGATGGTAACA427050.11586896022361447No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGGG419970.11394798554059564No Hit
AGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCC418590.11357355827187163No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA413910.11230376144750326No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC411670.11169599544609617No Hit
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATACAAGGCCA411090.11153862746358899No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC408370.1108006258904518No Hit
GGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAACAGTTTCCC402050.10908585752933897No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA394740.1071024783015328No Hit
AATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTT388110.10530359946701093No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG387050.10501599591277364No Hit
GATATGTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATG386940.10498615026091883No Hit
GAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAAA386190.10478265718009057No Hit
ATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAACAAG382430.10376247853487153No Hit
GATATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATG376360.10211554120070143No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC374840.10170312855688948No Hit
GTAATATGTTAATTATTGTAGAACTGGCTGGAAGGTAACTTCCTGTCGGC374640.10164886373533527No Hit
AATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCT374410.10158645919054793No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG374240.10154033409222687No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTTGGTTTGTGCAAGCTTACCTTG372790.10114691413595889No Hit
GAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGG372120.1009651269837523No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGGGATGGTAAC371660.10084031789417763No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC369470.10024611809815909No Hit
GGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGA368940.10010231632104045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)