FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-087_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-087_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52651320
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG6648111.2626672987495848No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG4634250.8801773630746579No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA3906570.7419700018916905No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA3439180.6531991980447973No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC2852570.5417850872494745No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA2582030.4904017601078187No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC2385150.4530085855397358No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT2384020.452793966039218No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC2269490.4310414249823176No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC2139740.4063981681750809No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT2058110.3908942833721928No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT1917060.3641048315597786No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT1838550.3491935244928332No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT1582740.30060784800836904No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG1532300.2910278412772937No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA1512360.28724066177258234No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT1506170.2860650027387727No Hit
TGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTA1470030.27920097729743526No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA1456930.2767129105215216No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG1448070.2750301416944532No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTA1412920.2683541457270207No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA1338870.2542899209364552No Hit
CTCAAAAGGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCA1259370.2391905843956049No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG1251300.23765785929013744No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA1235450.2346474884200434No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC1210450.2298992693820402No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT1210240.22985938434212094No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1101220.2091533507611965No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC1098760.20868612600785696No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTATTTGGGGTTTCGGCCTTGA1093420.20767190642133948No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT1084340.20594735326673672No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA1070560.20333013493298935No Hit
TTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTT1055290.20042992274457694No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT1026780.1950150537536381No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT1020380.19379950967990928No Hit
CCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTC1004730.19082712456211925No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA1004410.1907663473584328No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT954580.18130219717188478No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA948560.1801588260275336No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC945780.17963082407050762No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA945450.179568147579206No Hit
TCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAA942860.17907623208686885No Hit
GTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCAC937380.17803542247373855No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG918240.17440018597824328No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC914800.17374683103861405No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA910310.17289405089938864No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA906420.17215522801707533No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT892760.1695608011347104No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT863970.16409275209054588No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTTTAGCTTAATCCA845110.16051069564827625No Hit
CTCGGGGTGCGTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATC827990.15725911525105163No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG826070.15689445202893298No Hit
CCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC817970.15535602906061996No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC812920.1543968888149433No Hit
CTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGT811100.15405121846897665No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT807670.1533997628169626No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG805990.15308068249760878No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT804960.15288505587324305No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA802980.1525089969254332No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC802200.1523608524914475No Hit
TGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAG792680.15055273068177588No Hit
GCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAG789950.1500342251628259No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG788220.1497056484053961No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC778500.14785954084342046No Hit
CTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCA776320.14744549614330657No Hit
GCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTTTAGCT775080.1472099844790216No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG759820.14431167157822444No Hit
TTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTG753190.143052443889346No Hit
GCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCTG751090.14265359349015372No Hit
TGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCT748270.14211799438266695No Hit
CTTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATG738180.14020161317892885No Hit
CGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTTTAGC713640.1355407613712249No Hit
TTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAA700620.1330678888962328No Hit
GCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCT699030.1327659021654158No Hit
TTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGGAAAGCTCTCC696720.1323271667263043No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT683890.12989038071600104No Hit
CTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAA674120.12803477671594937No Hit
TGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCAT671400.12751817048461464No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC662260.12578222160432065No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGCCTTGAA658670.1251003773504634No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT653370.12409375491440672No Hit
CGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGC652750.12397599908226423No Hit
TTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGC642630.12205392001568052No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT629070.11947848600946757No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC624450.11860101513124457No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG621760.11809010676275541No Hit
CGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTACGATTCAGCT621600.11805971816091221No Hit
TTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAAG608960.11565901861529777No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC603140.11455363322325063No Hit
TGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACA601660.11427253865620084No Hit
GTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAAC596510.11329440553437217No Hit
CCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATT594140.11284427436956945No Hit
TTTTAAGCAACGCTTTTAGCTTAATCCACATAACTGATACTTCGTATCGA591570.11235615745246272No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA588780.11182625620782156No Hit
GTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTATACGCAC584090.11093549031629217No Hit
GCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTC576210.10943885167551354No Hit
TTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAG575920.10938377233467272No Hit
CCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACATATATACAA575440.10929260652914304No Hit
CCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCA574000.10901910911255407No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT571950.1086297551514378No Hit
GCGGCAAATACGATATATTCTTACGTGGGACTGAATTCGGTTGATTTGCT568970.10806376744210781No Hit
CTGATACTTCGTATCGACTTTGTGCGCATTTTGCTATTATGTGTTCTTTT565910.10748258543185622No Hit
TTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCACATAAC563010.10693179202344784No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTATTTGGGG558370.10605052256999443No Hit
CTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC553100.10504959799678337No Hit
AGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAAT551600.10476470485450318No Hit
TGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTATACGCACA542700.10307433887697404No Hit
TGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCT539560.10247796256580083No Hit
TCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAG539010.10237350174696475No Hit
CTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTCTATAGCAAACCTT536040.10180941332524997No Hit
TTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGA534300.10147893728020493No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA530890.1008312802034213No Hit
CGCTGAATCAATTCAGGCAAATTATTTATTTGGGGTTTCGGCCTTGAATT530420.10074201368550685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)