FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-086_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-086_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22649403
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATACAAGGCCA854950.37747131789742977No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA588430.2597993421725067No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA536230.2367523770935596No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT501960.22162173546031216No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG448940.19821272993376468No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA426780.1884288075937366No Hit
AATGGATCCGTCCATTTTTAGTGTGTATCAATCGAGTTTCTGACCTATCA417890.18450375932645993No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA413160.18241540406164347No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT408230.18023874624863181No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC406200.17934247538445053No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG403920.17833582633502526No Hit
GCCGAATTTAAGCATATAATTAAGCGGAAGAAAAGAAAATAACAATGATT400060.1766315871548579No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG392690.1733776382538648No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC388890.171699889838156No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT380730.16809714587179186No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG370190.16344360158190482No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA369630.16319635444695826No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT354100.15633966157960102No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA353030.15586724294675672No Hit
AAAGAAGTGACACGTAATGGATCCGTCCATTTTTAGTGTGTATCAATCGA333610.1472930655170028No Hit
GGGGAATTAGAGTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCAC322540.1424055194743985No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT314970.13906326802521018No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCACATCTAAGGAAG312360.13791091977126285No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT310930.13727955655166718No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA307580.13580048886939758No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG303780.1341227404536888No Hit
CTGAGAAATAGCTACCACATCTAAGGAAGGCAGCAGGCGCGTAAATTACC301370.1330586947479366No Hit
CAGCAGGCGCGTAAATTACCCAATTCTAAAGAAGAGAGGTAGTGACAAGA290050.12806077052008832No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG284850.12576490426701314No Hit
GGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGA284310.12552648738688607No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA279010.12318646985971331No Hit
GTTCATTTCAGTCACGTTTAGGGGCGAAAGACTAATCGAAAAGCCTATTA275720.12173389294190227No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA275670.12171181730485346No Hit
TAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAAA274740.12130121045574578No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA265560.11724812349358613No Hit
AGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAG262820.1160383785833119No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA262580.11593241552547764No Hit
CCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTG260720.1151112018272623No Hit
GAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAG258580.11416636456157364No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG258500.11413104354229557No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT256290.11315530038473862No Hit
CAAAGAAGTGACACGTAATGGATCCGTCCATTTTTAGTGTGTATCAATCG254760.11247978589104535No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT250030.11039143062622886No Hit
GGGAATTAGAGTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCACA247490.10926998826414983No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA237850.10501380544114122No Hit
GACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGG236110.10424557327184296No Hit
GCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATACC232290.10255899460131467No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC230990.10198502803804585No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT230100.10159208169857722No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA229000.10110641768350363No Hit
CTTGGGGGCATTTGTATTCAGATGTCAGAGGTGAAATTCTTAGATTTTCT228850.10104019077235722No Hit
CTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTT228560.10091215207747418No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)