FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-083_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-083_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45667861
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1779120.38957813242008427No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1257660.27539279757376856No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1123660.2460504992778182No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT1104270.24180462491991905No Hit
GTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGG1102470.24141047464430182No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA1044740.2287691994157554No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG1013300.22188470793497425No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA982240.2150834259568233No Hit
GTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTG963200.21091419193029426No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT956670.209484302319305No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT952390.20854710055283734No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT907730.19876779427002284No Hit
GAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATC902600.1976444659845137No Hit
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT829040.18153685805428899No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA797380.17460419265093235No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGC793230.17369545729325925No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC782390.1713217967445421No Hit
GGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCA774970.16969702171949766No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG773000.1692656461400721No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAG720150.15769295610319914No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA714680.1564951772100734No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA707510.1549251452788647No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC706980.15480908991993295No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC688380.15073620373855479No Hit
GTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCT671250.14698520694893066No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA664990.14561443987928402No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA661570.14486555435561127No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC661250.14479548319550153No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA650490.14243934043681178No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG646560.14157877900171412No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGA631430.13826572696277586No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAG626100.13709860420219813No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC617950.13531397934315337No Hit
GGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGG610200.1336169434342458No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG608120.13316148089353255No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT599350.13124109316177518No Hit
CCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCT598990.13116226310665174No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC584760.12804628620552208No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG584590.1280090609017138No Hit
GCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCG577210.1263930447716831No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC564110.12352450665469093No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT562630.1232004275391834No Hit
GACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAG557670.12211432455748256No Hit
GACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA557050.12197856168476996No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT554000.12131069593997407No Hit
CCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGAACGAGG531870.11646483727363538No Hit
GGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATA521970.11429701075774054No Hit
GTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGT520260.11392256799590415No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG512420.11220582457321573No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAA508390.1113233659005838No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC507750.11118322358036432No Hit
GACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCC507470.1111219113152683No Hit
GTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA507430.11111315242025457No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT507200.11106278877392572No Hit
GAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGA505730.11074089938217163No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC497460.10892999783808574No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC493310.10802126248041265No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC489020.10708187099019155No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG488450.10695705673624607No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG486690.10657166535564255No Hit
GTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGG484710.1061381000524636No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA483060.10577679563314778No Hit
GACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATT482330.10561694579914746No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGA481460.10542643983259911No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG480510.10521841607602335No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT480110.10513082712588619No Hit
GGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCT475440.10410822613303478No Hit
GCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCT473190.10361553828851322No Hit
GAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGC468740.10264111121823727No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG468680.10262797287571669No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT468350.10255571199185352No Hit
CGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTG466910.10224039177135973No Hit
CGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGC459570.10063313453634273No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT457530.1001864308906432No Hit
ATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG457520.10018424116688977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)