FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-082_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-082_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25636628
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG1976050.7707916969423592No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1684710.657149606414697No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA1500680.5853655948824471No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG1143030.445858168242719No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA1101470.42964698789559996No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC941870.36739231072042705No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA938510.36608168593779183No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG835330.3258345832377019No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT782930.305395077698986No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT746780.2912941592786696No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC743750.2901122565729003No Hit
TGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAA644840.251530739534076No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA632550.24673681733806802No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG618700.24133439077869367No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT612800.23903299607109016No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA602930.23518303577209923No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC600650.2342936832410253No Hit
CTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTT571510.22292713378686152No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA555350.21662365268942546No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT551980.2153091272377943No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT541440.21119782211607546No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT539490.21043719166186753No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT519500.20263975433898718No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA517840.20199224328566143No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT512910.20006921347066392No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG494930.19305581061596713No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC490040.19114838347695337No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT481810.18793813289329625No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG474760.18518816125115986No Hit
GAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAAA444960.17356416764326416No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA432760.1688053514682196No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA432030.16852060263151614No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT425710.1660553798260832No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA424730.16567311426448128No Hit
GAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGT414640.16173733924757966No Hit
AAGGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTA413860.1614330870658965No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG411140.16037210509900132No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA378740.1477339375521617No Hit
GAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTA375280.1463843060795671No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC373970.1458733184410992No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA373570.14571729168126168No Hit
GCCGAATTTAAGCATATAATTAAGCGGAAGAAAAGAAAATAACAATGATT365500.14256945180153957No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT360210.1405059979026883No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACAC358120.13969075808253723No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC355520.1386765841435933No Hit
CGGCGAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA352580.13752978745878747No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC352280.13741276738890934No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG352190.1373776613679459No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC351010.1369173824264252No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG350610.13676135566658768No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC339950.1326032425169176No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG339910.13258763984093383No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG338220.1319284267806203No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC338020.13185041340070153No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC322500.1257965751190055No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA321800.12552352828928984No Hit
TCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAG320540.12503204399580162No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC317760.12394765801493081No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC314280.12259022520434434No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA312690.12197001883399018No Hit
CGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTCATAGTGTTTC311560.12152924323744917No Hit
TCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAA307140.1198051475412445No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTGCCTTCTAT303190.11826438328784894No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG303170.11825658194985705No Hit
TTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACTT300300.11713708994802281No Hit
GTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAA299350.11676652639340869No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG295980.11545200094177752No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG295200.11514774876009434No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA293300.11440662165086611No Hit
CTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCT290290.11323252028308871No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA290040.11313500355819026No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA287720.11223004835113262No Hit
GCGGCAAATACGATATATTCTTACGTGGGACTGAATTCGGTTGATTTGCT286260.11166055067772564No Hit
TCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCAT284480.11096623159644865No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC283580.1106151713868142No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA283190.11046304529597263No Hit
ACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATAC282370.11014319043830569No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT281740.10989744829156159No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA278120.10848540611503198No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT277970.1084268960800929No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT274620.10712017196645363No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT274420.10704215858653486No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA270320.10544288429820022No Hit
AGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAAT266510.1039567294107478No Hit
CTGATACTTCGTATCGACTTTGTGCGCATTTTGCTATTATGTGTTCTTTT264900.10332872170240173No Hit
GGCAAATACGATATATTCTTACGTGGGACTGAATTCGGTTGATTTGCTTA260990.10180356012498991No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTAT258970.1010156249878104No Hit
GCTGGTTATTTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATA257450.10042272330042781No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT257280.10035641192749686No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA256530.10006386175280149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)