FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-076_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-076_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32710862
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG4662471.425358341213998No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA3045620.9310729873153449No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG2649510.809978654796685No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA2462700.752869184554048No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC1850080.5655858289518632No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC1842490.563265498781414No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT1768380.5406094159181741No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA1643090.5023071541190202No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC1635830.5000877078690253No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC1606510.49112432439108455No Hit
CCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATT1596410.4880366650074829No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA1475860.4511834631566725No Hit
TGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTA1368980.4185093012834697No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA1309370.40028599674322246No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT1285400.39295815561204106No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT1129950.34543571490106256No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT1121990.34300227245616455No Hit
GCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCT1095120.33478787566038465No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG1035080.31643311631469695No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC1017000.31090590030920007No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT1013760.3099154036356486No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT994390.30399382321383034No Hit
CGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGC966190.29537283364773453No Hit
TTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCACATAAC961230.2938565177524212No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG956900.29253279843252067No Hit
TCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAA945460.2890354891901045No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT930300.28440094302620333No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG923540.28233435120113926No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG877460.26824728739951886No Hit
TTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTT868570.26552953572424964No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA862090.2635485423771468No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT861380.2633314890937451No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA832980.25464935775767694No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC832240.2544231332087794No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT826640.2527111636495547No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA787440.2407273767349818No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT781860.2390215213527543No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG771830.23595526158864294No Hit
ACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAAT758220.2317945641420272No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT747280.2284501093245418No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC740750.22645383053494583No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT733100.22411515783350497No Hit
GTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCAC731620.22366270873570987No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA715500.21873468207594163No Hit
CAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTG669690.20473015966378386No Hit
GACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAA624950.19105274572097794No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA622600.19033432992380328No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG621650.19004390651643482No Hit
GGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGA617110.18865598833806338No Hit
CTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGT616910.18859484656809103No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA612570.1872680701596919No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA607870.18583123856534262No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT596210.1822666733759569No Hit
GGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTG594370.18170416909221163No Hit
CCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTC594350.1816980549152144No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA591010.1806769873566768No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC587420.17957949258567385No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC572230.17493577515627684No Hit
CTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTT570230.17432435745655372No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT567770.1735723136858943No Hit
GCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTC558230.17065585125821509No Hit
TCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAG555570.16984266571758336No Hit
TTTTAAGCAACGCTTCTAGCTTAATCCACATAACTGATACTTCGTATCGA549400.16795644211393757No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA542180.16574922421793714No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT541400.16551077131504513No Hit
ACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATAC536270.16394248491525537No Hit
CGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTACGATTCAGCT521290.1593629663443293No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG500710.15307147821417852No Hit
TTTTGAGTGTAATAGAGATGGTAACATACATACAAATGAACTCCTTCACA496300.15172330218628907No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG492630.15060135070729716No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA490520.1499563050340893No Hit
GCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCTG486350.14868149913016662No Hit
GCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTC485390.1483880186342995No Hit
TGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACA481600.14722938209332423No Hit
TGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAG476110.1455510405075843No Hit
GCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAG473280.1446858844624761No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC463710.14176025076930102No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATA455100.13912809757199307No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC454900.13906695580202075No Hit
TCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTA448300.1370492773929345No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG446820.13659682829513942No Hit
CTCGGGGTGCGTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATC427320.1306355057228391No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA424720.12984066271319905No Hit
TCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCAT423220.12938209943840673No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA423100.12934541437642336No Hit
CTGATACTTCGTATCGACTTTGTGCGCATTTTGCTATTATGTGTTCTTTT417370.12759370266671663No Hit
TTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTC415050.12688445813503782No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA409870.125300886292755No Hit
CTTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATG407940.12471086821252218No Hit
CTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAA403770.1234360623085995No Hit
TGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCT402070.12291635726383487No Hit
TTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAG399490.12212762843119207No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA396350.12116770264262679No Hit
GGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTT389940.11920810891501421No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT384160.11744111176281444No Hit
TTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGGAAAGCTCTCC382620.11697032013402765No Hit
CTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCA375550.11480895856550648No Hit
GCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGAGTGTGT371900.11369312126351179No Hit
CTTGCTTTAGCACTCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCT370380.11322844381172223No Hit
TTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGC365450.11172129918190477No Hit
TTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACTT364300.111369734004564No Hit
TGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCT363940.11125967881861383No Hit
TTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAAG355670.10873146663025877No Hit
GCGGCAAATACGATATATTCTTACGTGGGACTGAATTCGGTTGATTTGCT353730.10813839146152737No Hit
TGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCAT348850.10664653227420298No Hit
CTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTC347650.10627968165436912No Hit
CCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTT333150.1018469033313766No Hit
CTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAA332290.10158399372049566No Hit
GTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAAC331630.10138222587958703No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTTTTT328630.10046509933000237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)