FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-070_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-070_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58557447
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG6587521.1249670772019824No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT4530050.7736078384701437No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG4334210.7401637574807521No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTA4023380.6870825498932698No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA3890020.6643083329776996No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA3475890.5935863289941585No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC2968100.5068697752482276No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC2597580.4435951587848425No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC2260740.38607215919095655No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA2084740.3560162040534315No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC2045000.3492297060013562No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT1754920.29969202721559907No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG1721750.2940275043070098No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA1659640.2834208260479662No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT1645420.2809924414908321No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG1613010.2754577056612458No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT1573030.2686302222158012No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT1552340.26509693976241827No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG1542230.2633704300667343No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA1539160.2628461585765513No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGGATA1437170.24542907411930032No Hit
GTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTATACGCAC1289500.2202111031240826No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA1261720.21546704384158005No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA1200940.20508749297079154No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT1185550.20245930462098183No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG1176370.20089161332460412No Hit
TGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTATACGCACA1097290.18738692620940253No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT1087010.1856313851934153No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA1086530.18554941440667658No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT1084470.18519762311358964No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA1061810.1813279188896333No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC1047220.1788363485177214No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1042700.1780644569425986No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCTCTGA1015880.17348433923357348No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT963480.16453586168126488No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG954010.1629186463678992No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC949440.1621382161691578No Hit
TGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAG929380.15871252037336941No Hit
TCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAA925600.1580670004278021No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG923990.15779205674728272No Hit
TTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTT915160.1562841358162353No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT906290.15476938398629297No Hit
TTTTGAGTGTAATAGAGATGGTAACATACATACAAATGAACTCCTTCACA887310.15152812246066671No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGGAT884820.15110289900445967No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT865410.1477882053157133No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA857170.14638104014336556No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT840100.14346595403997034No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA837480.14301853016235494No Hit
CTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGT836970.14293143620144505No Hit
TTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGGAAAGCTCTCC830360.14180263015906414No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA826660.14117077201128664No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT823920.14070285543698652No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC806740.13776898436163038No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG791840.1352244745232831No Hit
GCGGCAAATACGATATATTCTTACGTGGGACTGAATTCGGTTGATTTGCT785050.13406492943587517No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT770920.13165191440125457No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC768290.1312027827989154No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT756270.12915009768100033No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA743290.12693347098960786No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC739510.12628795104404056No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG733900.12532991747403194No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC728240.12436334528040474No Hit
CCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTC726790.12411572519546489No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT719320.12284005482684379No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC711170.12144825917700953No Hit
CTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTGGTAATGCAAATTAT707340.12079420060782363No Hit
TTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTG699190.11940240495798937No Hit
TTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAA699130.11939215860964703No Hit
TCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAG698440.11927432560371015No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA692670.11828896843812198No Hit
CCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATT691660.11811648824102594No Hit
TCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCT685190.11701159034477715No Hit
GCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTC675440.11534655873914723No Hit
TTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCACATAAC671240.11462931435518356No Hit
GCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCT665380.11362858766708188No Hit
CTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCA655060.11186621575219972No Hit
CTGGAGTTGACTAATATAATTTTACCAGGTAGAGATAATGATTAGAGGAA654440.11176033681932888No Hit
TGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCT650100.1110191842892331No Hit
GCTGGTTATTTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATA649760.11096112164862652No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG648650.11077156420429327No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC642080.10964958906080724No Hit
GCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAG640840.10943783119506559No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA618180.10556812697110923No Hit
TTTTAAGCAACGCTTCTAGCTTAATCCACATAACTGATACTTCGTATCGA615080.1050387323067551No Hit
TTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTTG614610.10495846924474012No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA599930.10245152935031475No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG594710.10156009704453133No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTAT592000.10109730364440239No Hit
GCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCTG589310.1006379256937209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)